고속대량 스크리닝

고속대량 스크리닝(영어: high-throughput screening, HTS)은 특히 약물 발견에 사용되며 생물학, 재료과학^[1] 및 화학 분야와 관련된 과학적 실험 방법이다.^[2]^[3] 로봇공학, 데이터 처리/제어 소프트웨어, 액체 취급 장치 및 민감한 검출기를 사용하는 고속대량 스크리닝을 통해 연구자는 수백만 건의 화학, 유전학 또는 약리학 테스트를 신속하게 수행할 수 있다. 이 과정을 통해 특정 생체분자 경로를 조절하는 활성 화합물, 항체 또는 유전자를 신속하게 인식할 수 있다. 이러한 실험의 결과는 약물 설계 및 비상호작용 또는 특정 위치의 역할을 이해하기 위한 출발점을 제공한다.

같이 보기

약물 발견

각주

↑ Zhao, Yicheng; Heumueller, Thomas; Zhang, Jiyun; Luo, Junsheng; Kasian, Olga; Langner, Stefan; Kupfer, Christian; Liu, Bowen; Zhong, Yu; Elia, Jack; Osvet, Andres; Wu, Jianchang; Liu, Chao; Wan, Zhongquan; Jia, Chunyang; Li, Ning; Hauch, Jens; Brabec, Christoph J. (2021년 12월 16일). “A bilayer conducting polymer structure for planar perovskite solar cells with over 1,400 hours operational stability at elevated temperatures”. 《Nature Energy》 (영어) 7 (2): 144–152. Bibcode:2022NatEn...7..144Z. doi:10.1038/s41560-021-00953-z. ISSN 2058-7546. S2CID 245285868.
↑ Inglese J and Auld DS. (2009) Application of High Throughput Screening (HTS) Techniques: Applications in Chemical Biology in Wiley Encyclopedia of Chemical Biology (Wiley & Sons, Inc., Hoboken, NJ) Vol 2, pp 260–274 doi/10.1002/9780470048672.wecb223.
↑ Macarron, R.; Banks, M.N.; Bojanic, D.; Burns, D.J.; Cirovic, D.A.; Garyantes, T.; Green, D.V.; Hertzberg, R.P.; Janzen, W.P.; Paslay, J.W.; Schopfer, U.; Sittampalam, G.S. (2011). “Impact of high-throughput screening in biomedical research”. 《Nat Rev Drug Discov》 10 (3): 188–195. doi:10.1038/nrd3368. PMID 21358738. S2CID 205477370.

더 읽을거리

Lin, Yibin (2018). “What's happened over the last five years with high-throughput protein crystallization screening?”. 《Expert Opinion on Drug Discovery》 13 (8): 691–695. doi:10.1080/17460441.2018.1465924. ISSN 1746-0441. PMID 29676184.
Staff (2008년 8월 1일). “High-Throughput Screening Challenges”. 《Genetic Engineering & Biotechnology News》. Drug Discovery Rountable Discussion 28 (14) (Mary Ann Liebert). 26–27면. ISSN 1935-472X. 2008년 10월 1일에 확인함.
Zhang XHD (2011) "Optimal High-Throughput Screening: Practical Experimental Design and Data Analysis for Genome-scale RNAi Research, Cambridge University Press"
Florian, Amy; Lepensky, C; Kwon, O; Sklar, L; Haynes, M (201). “Flow cytometry enbles a high-throughput homogenous fluorecent antibody-binding assay for cytotoxic T cell lytic granule exocytosis”. 《Journal of Biomolecular Screening》 4 (18): 420–429. doi:10.1177/1087057112466697. PMC 4043149. PMID 23160568. Flow cytometry enables a high-throughput homogeneous fluorescent antibody-binding assay for cytotoxic T cell lytic granule exocytosis
Florian, Amy E.; Lepensky, Christopher K.; Kwon, Ohyun; Haynes, Mark K.; Sklar, Larry A.; Zweifach, Adam (2013). “Flow Cytometry Enables a High-Throughput Homogeneous Fluorescent Antibody-Binding Assay for Cytotoxic T Cell Lytic Granule Exocytosis”. 《Journal of Biomolecular Screening》 18 (4): 420–429. doi:10.1177/1087057112466697. ISSN 1087-0571. PMC 4043149. PMID 23160568.

외부 링크

Open Screening Environment
Setting up High-Throughput Screening Laboratory (Koppal, Lab Manager Magazine)
Assay Guidance Manual (NIH, NCGC)
OncoSignature High Throughput Screening

[1] Zhao, Yicheng; Heumueller, Thomas; Zhang, Jiyun; Luo, Junsheng; Kasian, Olga; Langner, Stefan; Kupfer, Christian; Liu, Bowen; Zhong, Yu; Elia, Jack; Osvet, Andres; Wu, Jianchang; Liu, Chao; Wan, Zhongquan; Jia, Chunyang; Li, Ning; Hauch, Jens; Brabec, Christoph J. (2021년 12월 16일). “A bilayer conducting polymer structure for planar perovskite solar cells with over 1,400 hours operational stability at elevated temperatures”. 《Nature Energy》 (영어) 7 (2): 144–152. Bibcode:2022NatEn...7..144Z. doi:10.1038/s41560-021-00953-z. ISSN 2058-7546. S2CID 245285868.

[2] Inglese J and Auld DS. (2009) Application of High Throughput Screening (HTS) Techniques: Applications in Chemical Biology in Wiley Encyclopedia of Chemical Biology (Wiley & Sons, Inc., Hoboken, NJ) Vol 2, pp 260–274 doi/10.1002/9780470048672.wecb223.

[3] Macarron, R.; Banks, M.N.; Bojanic, D.; Burns, D.J.; Cirovic, D.A.; Garyantes, T.; Green, D.V.; Hertzberg, R.P.; Janzen, W.P.; Paslay, J.W.; Schopfer, U.; Sittampalam, G.S. (2011). “Impact of high-throughput screening in biomedical research”. 《Nat Rev Drug Discov》 10 (3): 188–195. doi:10.1038/nrd3368. PMID 21358738. S2CID 205477370.

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